ASSESSMENT OF THE TOXIC EFFECTS OF DIFFERENTLY PROCESSED NIGELLA SATIVA BASED DIETS ON THE HAEMATOLOGICAL INDICES OF ALBINO RATS

  • L. O. Oyekunle Department of Science Laboratory Technology, Oyo State College of Agriculture and Technology, Igboora, Oyo State, Nigeria.
  • M. O. Raji Department of Animal Health Technology, Oyo State College of Agriculture and Technology, Igboora, Oyo State, Nigeria
  • T. O. Olaniyi Department of Science Laboratory Technology, Oyo State College of Agriculture and Technology, Igboora, Oyo State, Nigeria.
  • A. I. Amuzat Department of Science Laboratory Technology, Oyo State College of Agriculture and Technology, Igboora, Oyo State, Nigeria.
Keywords: Nigella sativa, heamatological parameters, neutrophils, lymphocytesase soil quality.

Abstract

The effects of differently processed Nigella sativa seed-based diets on haematological indices of albino rats were investigated. The aim was to test if N. sativa seeds processed using different methods would have toxic effects on some haematological parameters of albino rats; adopting completely randomized design. The experimental animals were fed with diets supplemented with 10% raw (RAN), Parboiled (PAN), Boiled (BON), Roasted (RON) and control diet at 0% supplementation. The animals were fed for six weeks before being sacrificed and blood collected from them for analysis. The white blood cell count (WBC), red blood cell count (RBC), haemoglobin (Hb), packed cell volume, mean corpuscular haemoglobin, Mean corpuscular haemoglobin concentration, mean corpuscular volume, monocytes, neutrophils, lymphocytes, eosinophils and Basophils were determined using standard analytical techniques ans Analysis of variance (ANOVA) as the tools for analysis with the mean separation. The results showed that all treatment groups have higher values (concentrations) of PCV, Hb and WBC than the control group. The animal group that received PAN had highest concentrations of PCV (47.30±2.40), RBC (18.7±0.60) and Hb (19.98±0.70) while the group fed with BON showed least concentration of PCV (45.65±2.92), RBC (7.6±1.63) and Hb (18.92±9.02). RAN with 12.43±8.02 x 103mm3 white blood cells had the highest concentration of WBC while the PAN with 6.81±0.70 x103mm3 white blood cells had the lowest concentration of WBC among the treatment groups. The calculated values for neutrophil/lymphocyte ratio (NLR) are as follow in descending order 0.65, 0.63, 0.58, 0.58 and 0.54 for RON, RAN, PAN, NOD and BON respectively. These values were not too high to cause any major damage to the organisms that were fed with the different formulated diets. The results showed that Nigella sativa seeds, processed in all the methods assessed, has no toxic effect on the haematological parameters of albino rats.

Published
2024-08-30
Issue
Vol 19 No 1 (2024): Vol. 19
Section
Articles